Domain Of Unknown Function

We combined high throughput ligand screening results for transport system solute binding proteins with the synergetic analysis.

Domain of unknown function. The structure of r39 is composed of three domains. These families have been collected together in the pfam database using the prefix duf followed by a number with examples being duf2992 and duf1220. A large number of genes with duf domain of unknown function from different species have been deposited in genbank. This is surprising as a large number of dufs are shared between bacteria and other domains of life.

Recently numerous proteins containing the domain of unknown function duf 3494 were found to bind ice crystals and hence are classified as ibps. They can be found in different organisms including bacteria yeasts and microalgae supporting the hypothesis of horizontal transfer of its gene. However duf288 proteins do not affect csc assembly suggesting that duf288 proteins might not directly mediate cellulose. A domain of unknown function duf is a protein domain that has no characterised function.

In this thesis i identify duf288 domain of unknown function 288 proteins as novel cell wall synthesis related proteins by co expression analysis and reverse genetics. The r39 active site is located in an extended groove between a five stranded antiparallel β sheet. Although the 3d structure is always a discontinuous β solenoid with a triangular cross section and an adjacent alpha. Search results results of search on crystallography journals online 15 articles match your search domain of unknown function duf results 1 to 10 sorted by relevance.

The latter two domains are inserted in the penicillin binding domain between the sxxk and sxn motifs in the way of matryoshka dolls. Mutations in duf288 proteins cause cellulose deficiency in both primary and secondary cell wall. These duf domains are either encoded by genes of known function or by genes of unknown function. The medium and long isoforms contain a unique n terminal domain of unknown function duf959 and our sequence based secondary structure predictions indicated that duf959 could be an intrinsically disordered domain.

A penicillin binding domain and two domains of unknown functions figure 771 1. S1 in the supplemental material. Domains of unknown function occur in large numbers in all kingdoms of life ranging from about just over 1 500 in eukaryotes to 2 704 in bacteria see table s1b and fig. These families have been collected together in the pfam database using the prefix duf followed by a number with examples being duf2992 and duf1220 there are now over 3 000 duf families within the pfam database representing over 20 of known families.

Circular dichroism spectroscopy confirmed the disordered character of duf959 and static light scattering indicated a monomeric state for glycosylated duf959. Duf3494 ibps constitute today the most widespread of the known ibp families. Given their biological importance large scale strategies are required to accomplish their functional assignments. Domain of unknown function duf families constitute 3 892 of the 16 295 families in the pfam database release 29 0.

For example duf1943 has been found encoded by the hypothetical protein genes of dro. The penicillin binding domain shares its overall fold with other penicillin binding proteins and the serine β lactamases. However dufs represent a much greater proportion of domains in bacteria than they do in other kingdoms with about one third of all detected domains being dufs.